Application

Immunofluorescence: ≤0.3 µg/10⁶ cells.
ELISA:

Optimal working dilutions must be determined by the end user.

Research Sub CategoryFragment Specific Secondary Antibodies

Mouse anti-Rat IgG2b γ2b chain Antibody, Cy5 conjugate detects level of Rat IgG2b γ2b chain & has been published & validated for use in ELISA & IF.

Research CategorySecondary & Control Antibodies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgG is one of the most abundant protein in human serum. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants.

Immunogen

Epitope: IgG2b γ2b chain

Prepared from rat IgG2b γ2b chain.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified by affinity chromatography. Liquid in 1.0 mL PBS/NaN₃.

Affinity Chromatography

Specificity

The antibody reacts with the γ2b heavy chain (Fc) of rat IgG2b.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Do not freeze.